five

RNA-seq of etv2 mutant knock-in heterozygous and homozygous zebrafish embryos

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NIAID Data Ecosystem2026-04-25 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP226227
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资源简介:
Zebrafish etv2^Gt(2A-Gal4)ci32 line was generated using CRISPR-Cas9 mediated NHEJ approach which has 2A-Gal4 sequence inserted with the exon 5 of etv2 gene thus interrupting etv2 coding sequence. Heterozygous and homozogous embryos were subjected to bulk RNA-seq Overall design: 3 batches of 15-20 heterozygous and homozygous embryos were frozen at the 15-somite and 24 hpf stages. Total 12 samples were sequenced: triplicate samples of Heterozygous and homozygous embryos at 15-somite and 24 hpf stages
创建时间:
2020-01-19
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