Effect of elastin-deficiency on gene expression in murine aortic smooth muscle cells at embryonic day 15.5

Defective elastin induces aortic smooth muscle cell hyperproliferation. As elastin is expressed from embryonic day 14 in the murine aorta, we utilized bulk RNA-seq to reveal molecular differences between Eln(+/+) and Eln(-/-) aortic smooth muscle cells at embryonic day 15.5. Overall design: Acta2-GFP, Eln(+/-) mice were bred with each other and embryos were collected at embryonic day 15.5. Aorta was isolated from Acta2-GFP embryos carrying Eln(+/+) or Eln(-/-). After digestion with collagenase enzyme mixtures, GFP+, CD31-, DAPI- smooth muscle cells were sorted by FACS and incubated with RNA lysis buffer. Extracted RNA was subjected to bulk RNA-seq.