Figure 3. Propagation of aggregated α-synuclein following inoculation of pre-formed fibrils in vivo.

Protein extract from the striatum of PFF- and PBS-inoculated mice were extracted and quantified with immunoblots. The levels of pS129 and total human aSyn found in the detergent-soluble (RIPA) fractions from PFF-1 and PFF-2 samples were expressed as the percentage fold change relative to PBS samples. The levels of pS129 and total human aSyn found in the detergent-insoluble fractions (UREA solubilization of RIPA-insoluble pellets) were expressed in the same way as the detergent-soluble fractions (RIPA).