Assessment of the molecular mechanism associated with osteogenic and angiogenic differentiation induced by FeMnSi scaffold. Assessment of the molecular mechanism associated with osteogenic and angiogenic differentiation induced by FeMnSi scaffold

To investigate the molecular mechanism underlying osteogenic and angiogenic differentiation induced by the De-HA scaffold, we conducted an in-depth gene microarray analysis on day 3 of MC3T3-E1 culture. We found that De-HA could enhance the transcription of osteogenic and angiogenic genes by activating FAK and downstream MAPK/ERK signaling to induce MC3T3-E1 differentiation. More importantly, our findings indicated that EGFR, a kind of transmembrane glycoprotein, played a key role in the osteogenic differentiation of MC3T3-E1, suggesting that the activation of the FAK/MAPK/ERK signaling cascade might be driven by EGFR. Overall design: The scaffold samples were segregated into two groups: the first comprised of the original FeMnSi scaffold, while the second consisted of a De-HA scaffold that had been dealloyed and coated with bioactive materials. MC3T3-E1 were seeded on each scaffold at a density of 1 × 105 per/well in 24-well plates and then cultured for 3 days. An extensive gene microarray analysis was conducted on day 3 of MC3T3-E1 culture.