Massively parallel reporter assay for programmed ribosomal frameshifting on designed sequence libraries

We designed an oligonucleotide libraries containing a potential frameshifting site, cloned the library variants into a dedicated reporter construct, transfected and integrated these constructs in the genome of K562 cells, and performed FACSseq (as well as targeted RNA sequencing) to determine if and to what extent frameshifting occurs. Massively parallel reporter assay for programmed ribosomal frameshifting