Crosstalk between Auxin Signaling and Osmotic Stress Response in the Regulation of Leaf Growth of Arabidopsis thaliana

Genome-wide transcriptome analysis of Arabidopsis thaliana was performed to understand the role of auxin in the response of leaf growth to osmotic stress. We studied transcriptional changes in proliferating leaves of the seedlings grown in vitro on control medium, medium supplemented with 25mM mannitol, 0.1µM NAA and 0.1µM NAA + 25mM mannitol. Overall design: Total mRNA was obtained from the first leaf pair of plants grown on medium containing 25mM mannitol, 0.1µM NAA and the combination of NAA and mannitol at 10 DAS (days after stratification) and compared with leaves of plants grown on control medium. Three biological replicates were used for each treatment (3 replicates x 4 treatments = 12 samples).