Reproducible scRNA-seq PBMC Workflow: Reference, Toy Demonstration Data, and Analysis Outputs

This record contains a fully reproducible, containerized single-cell RNA-seq workflow for PBMC data analysis. The workflow implements: FASTQ quality control STARsolo alignment Seurat-based QC, normalization, clustering, and annotation Pseudobulk differential expression and TOST equivalence testing Cell-type–specific co-expression network construction Version 1 provides a lightweight demonstration bundle including: A chromosome 1 (chr1) GRCh38 mini-reference A pre-built STAR index for chr1 A reduced FASTQ subset (~100k reads) for rapid execution This demonstration dataset is designed to run in 5–10 minutes on a standard workstation and illustrates full pipeline functionality without requiring large memory resources. Future versions of this record will include the full GRCh38 reference, complete STAR index, and full-scale analysis outputs.