Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response. Doykov et al

This data relates to the paper 'Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response by I Doykov et al. Determining the protection an individual has to SARS-CoV-2 variants of concern (VoC) is crucial for future immune surveillance, vaccine development and understanding the changing immune response. We devised a more informative assay to current ELISA based serology using multiplexed, baited, targeted-proteomics for direct detection of multiple proteins in the SARS-CoV-2 anti-spike antibody immunocomplex.
Serum from individuals collected after infection, or first and second dose vaccination demonstrate this approach shows concordance with existing serology and neutralisation. Our assays show altered responses of both immunoglobulins and complement to the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.1) VoC. and a reduced response to Omicron (B1.1.1529). We were able to identify individuals who had prior infection, and observed that C1q is closely associated with IgG1 (r>0.82) and may better reflect neutralisation to VoC. Analysing additional immunoproteins beyond IgG, provides important information about our understanding of the response to infection and vaccination.
The skyine raw data files are provided and the processed data given in the data set which includes the ratio measurements of each protein measured in the immunocomplex assay used on samples from healthcare workers vaccinated and or infected to SARS-CoV2. Quality control assay data are also provided for each peptide/protein in a separate tab.

本数据集与由 I. Doykov 等人撰写的论文《基于定量、多路复用、靶向蛋白质组学的 SARS-CoV-2 变体特异性抗体反应确认》相关。确定个体对关注的 SARS-CoV-2 变体（VoC）的免疫力对于未来的免疫监测、疫苗研发以及理解免疫反应的变化至关重要。本研究设计了一种比当前基于 ELISA 的血清学检测更为信息丰富的检测方法，该方法采用多路复用、诱饵、靶向蛋白质组学技术，以直接检测 SARS-CoV-2 抗刺突抗体免疫复合物中的多种蛋白质。收集感染后或第一剂和第二剂疫苗接种后的个体血清，该技术显示出与现有血清学和中和反应的一致性。我们的检测结果显示，免疫球蛋白和补体对 Alpha (B.1.1.7)、Beta (B.1.351) 和 Delta (B.1.617.1) VoC 的反应发生改变，而对 Omicron (B1.1.1529) 的反应减弱。我们能够识别出先前感染过的个体，并观察到 C1q 与 IgG1 (r>0.82) 密切相关，可能更好地反映对 VoC 的中和反应。分析除 IgG 之外的免疫蛋白，为我们理解感染和疫苗接种的反应提供了重要信息。提供的数据集包括对样本进行免疫复合物检测的蛋白质比率的原始数据文件，以及每个肽/蛋白质的质量控制检测数据，分别位于单独的标签中。