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Pupylation sites on protein RutR

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We used RutR and specific biotin-DNA to validate the efficiency of SPIDER capturing protein-nucleic acid interaction. Pupylation sites on RutR after SPIDER assay was identified by LC-MS/MS analysis, by searching for an additional mass of ~243 Da, which represents the three C-terminal residues of Pup, i. e., GGE, that covalently binds to adjacent lysine by pupylation. )

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2021-06-04
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