Angptl-7 knockdown RNA sequencing of 4T1 orthotopic transplants into rat mammary fat pads

These samples are derived from tissues obtained from 4T1 spheroids transplanted orthotopically into the mammary fat pads of SRG OncoRats. 4T1 cell lines were established by viral transduction that stably express either a non-targeting control shRNA (NT4) or an shRNA targeting Angptl-7. 4T1 cells were cultured as spheroids in suspension for 24-hours prior to transplantation in 2% matrigel. 600,000 cells in a 1:1 mixture of matrigel and DMEM/F12 were transplanted orthotopically into the right T4 fat pads of SRG rats. At day 27 post transplanation, primary tumors. The primary tumors were cut in half to expose the cross-section, and the tumors were dissected to isolate the necrotic core region and the non-necrotic rim region. Samples were snap frozen and sent to Genewiz-Azenta for RNA extraction, library prep, and next-gen sequencing.本数据集样本源自SRG肿瘤大鼠（SRG OncoRats）乳腺脂肪垫原位移植的4T1细胞球（4T1 spheroids）所获取的组织。4T1细胞系通过病毒转导构建，可稳定表达非靶向对照短发夹RNA（shRNA，NT4）或靶向Angptl-7的短发夹RNA（shRNA）。移植前，将4T1细胞以悬浮培养方式制备为细胞球，培养24小时后置于2%基质胶（matrigel）中。将60万个细胞置于基质胶与DMEM/F12培养基按1:1体积比混合的体系中，原位移植至SRG大鼠的右侧T4脂肪垫。移植后第27天获取原位肿瘤组织：将肿瘤组织对半切开以暴露切面，随后通过解剖分离出肿瘤的坏死核心区（necrotic core region）与非坏死边缘区（non-necrotic rim region）。将样本快速冷冻后，送至Genewiz-Azenta进行RNA提取、文库制备与下一代测序（next-gen sequencing）。