Transcriptomic analysis of CIC and ATXN1L in normal human astrocytes

Aberrations in Capicua (CIC) have recently been implicated as a negative prognostic factor in a multitude of cancer types through activation of the MAPK signalling cascade and derepression of oncogenic ETS transcription factors. The Ataxin-family protein ATXN1L has previously been reported to interact with CIC in developmental and disease contexts to facilitate the repression of CIC target genes. To further investigate this relationship, we performed functional in vitro studies utilizing ATXN1LKO and CICKO human cell lines and characterized a reciprocal functional relationship between CIC and ATXN1L. Overall design: 3 biological replicates for each sample, two monoclonal knockouts per knockout condition. The parental line is a polyclonal normal human astrocyte immortalized with E6/E7 and overexpressing wildtype IDH1. CIC knockout monoclonal lines are labeled A2 and H9. ATXN1L knockout monoclonal lines are labeled B82 and B16