RNA sequencing of lncRNAs knockdown in human pancreatic cancer cell lines. Homo sapiens

We report the transcriptome changes that result from the transient knockdown of FAM83H-AS1 in Aspc1 cells and transient knockdown of LINC00673 in Panc1 cells. Overall design: Total RNA extracted 48 hour after transfection with targeting and control siRNAs. Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2500 (Illumina) instrument. Reads were mapped to the human genome (UCSC/hg19) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DESeq2.