Ejaculate investment differs by population, but not wing morph or perceived sperm competition risk, in Pacific field crickets

We raised crickets from two populations in Hawaii (O'ahu and Kaua'i) and two wing morphs (flatwing males, which cannot sing; and normal-wing males, which can sing), under conditions of perceived sperm competition risk (presence of male advertisement song) or its absence (quiet). In adulthood, males were euthanized, frozen, measured for body size and mass; their accessory glands (which produce seminal fluid) were dissected out and preserved for quantitative PCR (qPCR) analysis. We examined the effects of population, wing morph, and presence or absence of perceived sperm competition risk on male testes mass and production of three seminal fluid proteins (SFPs) implicated in male paternity success. The dataset includes a spreadsheet reporting data, including: male population of origin, wing morph, experimental acoustic treatment (song present or absent), body mass, pronotum width, and qPCR values for each of the three SFPs. It also includes R code used to analyze the data and generate the ..., , # Data from: Ejaculate investment differs by population, but not wing morph or perceived sperm competition risk, in Pacific field crickets

Dataset DOI: [10.5061/dryad.fttdz0969](https://doi.org/10.5061/dryad.fttdz0969)

## Description of the data and file structure

We studied seminal fluid expression in Pacific field crickets (*Teleogryllus oceanicus*) from Hawaii using quantitative PCR. We manipulated the presence and absence of song as a cue about sperm competition risk and replicated the study in males of two morphs (normal-wing and flatwing), populations (O'ahu and Kaua'i). We used a synthetic chorus; individual songs used to make the chorus, as well as MATLAB code to assemble a synthetic chorus, are provided.

### Files and variables

#### File: qPCR_Results\_Master\_wide.csv

**Description:** 

##### Variables

* ID: The cricket's ID number. Numbers beginning with O denote crickets from O'ahu while numbers beginning with W denote males from Wailua, Kaua'i.
* Plate: The number of...,