Cost-effective Detection of SNPs and Structural Variations in Full-length Genes of Wheat and Sunflower Using Multiplex PCR and Rapid Nanopore Kit

This study presents a target amplicon sequencing approach us-ing Oxford Nanopore Technologies (ONT-TAS) to rapidly sequence full-length genes and identify allele variants in sunflower and wheat collections. This procedure combines multiplex PCR and a rapid sequencing kit, significantly reducing the time and cost compared to previous methods. The efficiency of the approach was demonstrated by sequencing four genes (Ahasl1, Ahasl2, Ahasl3, and FAD2) in 40 sunflower genotypes and three genes (Ppo, Wx, and Lox) in 30 wheat genotypes.