Phase separation of phospho-HDAC6 drives aberrant chromatin architecture in triple-negative breast cancer [ChIP-seq, Cut&Tag, ATAC-seq]

To further elucidate the role of phospho-HDAC6 LLPS in transcriptional regulation, we performed RNA-seq experiments in TNBC cells treated with Nexturastat A or HDAC6 knockdown Overall design: Comparative gene expression profiling analysis of ChIP-seq data (HDAC6, Pan-acetyl, polII) and ATAC-seq for MDA-MB231 cells with and without HDAC6 inhibitor Nexturastat A treatment. Comparative gene expression profiling analysis of ChIP-seq data (HDAC6, Pan-acetyl, polII) and ATAC-seq for MDA-MB231 cells and its HDAC6 KD derivatives (shHDAC6).