RNA stability measurements in RBMS1 knockdown cells and control cells. RNA stability measurements in RBMS1 knockdown cells and control cells
收藏NIAID Data Ecosystem2026-03-11 收录
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In order to identify RBMS1-dependent changes in RNA stability, RBMS1 levels in SW480 breast cancer cells were stably knocked-down using short-hairpin RNAs. The cells were then treated with alpha-amanitin to inhibit transcription, RNA was isolated at 0 and 9 hours post-alpha-amanitin treatment, and the samples were transcriptomically profiled. Overall design: shRNAs targeting either RBMS1 (TRCN0000074936 and TRCN0000291051, Sigma) or a control shRNA were stably expressed in SW480 breast cancer cells. These cells were treated with 10ug/mL alpha-amanitin to inhibit transcription, and total RNA was isolated at 0 and 9 hours post-alpha-amanitin treatment.
创建时间：
2020-03-30
