Table 2 h AChE, h in Amaryllidaceae alkaloids from Narcissus pseudonarcissus L. cv. Dutch Master as potential drugs in treatment of Alzheimer's disease

Table 2 h AChE, h BuChE, POP and GSK-3β inhibitory activities of the tested Amaryllidaceae alkaloids isolated from Narcissus pseudonarcissus cv. Dutch Master expressed as IC50.
Compoundh AChE IC (μM) a 50h BuChE IC (μM) a 50SI for h AChE bPOP IC a (μM) 50GSK-3β (% inhib.) cGSK-3β IC a (μM) 50Homolycorine (1)64 ± 4151 ± 202.4173 ± 4154 ± 1n.d.Masonine (2)305 ± 34229 ± 240.8314 ± 3466 ± 427.9 ± 0.8Seco-isopowellaminone (3)294 ± 33> 500> 1.7> 50038 ± 1n.d.O -Ethyllycorenine (4)> 500> 500n.d.> 50058 ± 3n.d.N -Demethylmasonine (5)> 500474 ± 13<0.9> 500n.d.n.d.Narcimatuline (6)489 ± 605.9 ± 0.20.0129.2 ± 1.067 ± 320.7 ± 2.4O -Acetylpluviine (7)> 500> 500n.d.n.d.n.d.n.d.Tazettine (8)> 500> 500n.d.> 50049 ± 0n.d.Galanthine (9)606 ± 60> 500> 1.7> 50026 ± 7n.d.Galanthamine (10)1.7 ± 0.142.3 ± 1.324.9> 500n.d.n.d.Narwedine (11)281 ± 34911 ± 693.2907 ± 87n.d.n.d.Caranine (12)321 ± 42487 ± 551.5> 100058 ± 930.8 ± 0.3Lycoraminone (13)> 500> 500n.d.n.d.39 ± 1n.d.Lycoramine (14)456 ± 57> 500> 1.1> 500n.d.n.d.O -Methyllycorenine (15)> 500> 500n.d.> 500n.d.n.d.Lycorenine (16)> 500> 500n.d.> 50048 ± 3n.d.Oduline (17)> 500> 500n.d.252 ± 2758 ± 4n.d.Haemanthamine (18)> 500> 500n.d.> 50052 ± 0n.d.Tetrahydromasonine (19)> 500> 500n.d.> 50022 ± 0n.d.Hippeastrine (20)> 500> 500n.d.> 50011 ± 2n.d.Crinine (21)> 500> 500n.d.> 50040 ± 5n.d.Epimaritidine (22)> 500> 500n.d.n.d.n.d.n.d.Huperzine A c0.033 ± 0.001> 500> 15.151n.d.n.d.n.d.Berberine cn.d.n.d.n.d.142 ± 21n.d.n.d.Z -Pro-prolinal cn.d.n.d.n.d.2.75 × 10−3n.d.n.d.SB-415286 cn.d.n.d.n.d.n.d.n.d.70 nM
a Compound concentration required to decrease enzyme activity by 50%; the values are the mean ± SEM of three independent measurements, each performed in triplicate; b Selectivity index (SI) for h AChE is determined as ratio h BuChE IC / h AChE IC; c tested at 10 μM compound concentration; n.d. stands for not determined.