Summary of RIT-seq outputs following RNAi library selection in NHS.
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aAnnotations derived from GeneDB, http://www.genedb.org/Homepage/Tbruceibrucei927. b‘Tagged’ reads incorporate a 14-bp RNAi construct signature. cPlotted in Figure 2A. dHigh-confidence ‘hits’ characterised by >99 reads containing the 14-base RNAi construct signature, and >1 distinct RNAi target fragment. eBloodstream-form (BSF) T. b. brucei RNAi library fitness ratio derived from reference [24]; six-day induced read count divided by uninduced read count. fOther ‘hits’ are characterised by massive gain-of-fitness during RNAi induction (Tb927.8.6870) [24], a single RNAi target sequence (Tb927.5.1540, Tb927.6.2930), or <100 reads containing the 14-bp RNAi construct signature (relevant criteria highlighted in bold).
创建时间:
2014-05-15



