Summary of growth in the presence of quinolones.

For ease of comparison, the results of dilution plating (from Fig 4) were categorized in comparison to the wild-type dilution series on the same plate. The number in each entry indicates which dilution tube showed growth (more than one colony) relative to the wild-type series (+1 indicates that the mutant grew in the next more dilute spot than the wild type while -1 indicates that the mutant grew in the next more concentrated spot; note that each dilution step was five-fold, and so a reading of -3 would correspond to a dilution factor differential of 125). The downward arrow next to the number indicates that the colonies that did grow were noticeably smaller than the corresponding wild-type colonies on the same plate (or that no colonies grew in the corresponding mutant spot); the upward arrow for the ΔrecF spots indicated that the colonies grew larger than the corresponding wild-type colonies. Both negative numbers and downward arrows provide evidence for hypersensitivity.* Mutants that we designated as hypersensitive are indicated by an asterisk.a Ciprofloxacin, ciprob Nalidixic acid, nalSummary of growth in the presence of quinolones.