Comprehensive analysis of H3K9me2 oocytes, together with DNA methylation and expression analysis of G9a conditional KO oocytes and maternal KO preimplantation embryos

We report the first genome-wide landscape of H3K9me2 ChIP-seq profile in mouse oocytes. We also performed whole-genome bisulfite sequencing and RNA-seq analysis of G9a conditional KO oocytes and maternal KO preimplantation embryos. Our findings illuminate the functional importance of G9a in preimplantation development and, in addition, pose a question on the proposed role for H3K9me2 in protection of the maternal genome from active CG demethylation. ChIP-seq H3K9me2 profile was generated from wildtype fully-grown oocytes (FGOs). Whole-genome bisulfite sequencing anlaysis was performed on G9a conditional KO oocytes (growing oocytes, FGOs and MII oocytes) and maternal KO preimplantation embryos (early 2-cell embryos and blastocysts).