3,3',5-Triiodothyroacetic acid (TRIAC) induces embryonic ?-globin expression via thyroid hormone receptor a (RNA-seq)

Next Generation Sequencing Facilitates Quantitative Analysis of K562 cells treated with DMSO and TRIAC Overall design: mRNA profiles of K562 cells were generated by deep sequencing, in duplicate, using Illumina Navoseq 6000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using SYBR Green assays.