Gap-free telomere-to-telomere genome assembly of stone flounder (Kareius bicoloratus)

In this study, we successfully assembled the first gap-free telomere-to-telomere (T2T) genome of stone flounder combing Illumina short-reads sequencing, PacBio HiFi sequencing, Hi-C sequencing, and ONT ultra-long sequencing. The genome spans 587.80 Mb with a contig N50 of 25.64 Mb. 98.58% of the genome sequences were assembled onto 24 chromosomes without gaps. Telomeres were identified at both ends of 8 chromosomes and at one end of 15 chromosomes. We identified 128.10 Mb repetitive sequences and 22,312 protein-coding genes, among which 98.03% of the coding genes were functionally annotated.