Single cell RNA-seq analysis of bronchial epithelium in patients with severe SARS-CoV-2 pneumonia. Single cell RNA-seq analysis of bronchial epithelium in patients with severe SARS-CoV-2 pneumonia

To test whether airway epithelial and immune cells in patients with severe SARS-CoV-2 pneumonia exhibit specific gene expression signatures (namely interferon-response signature) we have performed bronchoscopy on intubated patients with severe SARS-CoV-2 pneumonia and obtained bronchial brushings. Overall design: Bronchial brushings from 4 patients with COVID-19 were obtained at Northwestern Memorial ICU. Single-cell libraries were prepared with 10x 5' V1 gene expression kit. Libraries were sequenced on Illumina NovaSeq 6000 and MiniSeq. Gene expression matrices were generated with 10x cellranger v3.1.0 software. ***RNA-Seq raw data is to be made available through dbGaP (controlled access) due to patient privacy concerns***