Control run hybridization. Enterovirus C

Laboratory strain poliovirus was hybridized to the array as a control run and a proof of concept. Degree of cross hybridization between polio nucleic acid and non-polio probes was evaluated. Specificity of the probe design was determined. Keywords: control study: target detection and specificity Overall design: 1 lab strain virus, poliovirus LS-C-1 was cultured on BGM cells. <24 hrs post infection, viruses were harvested and their nucleic acid was extracted. Viral RNA was labeled with Cy-3 dye and hybridized unto array.