No evidence for phenotypic condition-dependent ejaculate allocation in response to sperm competition in a seed beetle

Sperm competition is known to favour the evolution of male traits that confer an advantage in gaining fertilisations when females mate multiply. Ejaculate production can be costly and the strategic allocation of sperm in relation to the sperm competition environment is a taxonomically widespread phenomenon. However, variation among males in their ability to adjust ejaculate allocation has rarely been explored. Here, we manipulated the phenotypic condition of male seed beetles, Callosobruchus maculatus, via larval diet quality and measured ejaculate allocation across varying levels of sperm competition manipulated using olfactory cues. Further, we asked how strategic ejaculation was impacted by previous ejaculation. We found no variation in ejaculate allocation in response to experimentally manipulated cues to sperm competition. Ejaculate allocation was reduced by a male’s previous mating history, but was unaffected by the larval diets on which males were reared. We suggest that either m..., , , # Data from: No evidence for phenotypic condition-dependent ejaculate allocation in response to sperm competition in a seed beetle

[https://doi.org/10.5061/dryad.b2rbnzsr0](https://doi.org/10.5061/dryad.b2rbnzsr0)

## Description of the data and file structure

The excel file “weight.csv” contains the data and the R script “final.R” the code required to reproduce the analysis and figures presented in “No evidence for phenotypic condition-dependent ejaculate allocation in response to sperm competition in a seed beetle” by Blake W. Wyber, Joseph L. Tomkins & Leigh W. Simmons, and published in the journal Behavioral Ecology.

### Files and variables

#### File: weight.csv

**Description:** 8 columns

##### Variables

* ID: a unique identifier given to each male individual in the analysis
* Bean: the bean type on which the individual was raised, C=chickpea, M=mung bean
* CHC: the treatment level, being the number of male CHC blends coated onto the female that was then mated to the foca...