Systemic and mucosal immune responses induced by mucosal immunization of AFCo2 or PLc in BALB/c mice.

AFCo2 or PLc (100 µg) was administered by i.n or i.g route in BALB/c mice. Results of anti-PLc IgA in saliva and faeces and anti-PLc IgG in sera are expressed as the logarithm of the mean ± SEM. The ratio of IgG subclasses IgG2a/IgG1 is also reported. Vibriocidal activity of sera from immunized mice is also expressed as logarithm of the mean ± SEM. Statistical significance of the variance between multiple groups of experiments were calculated with one-way ANOVA, followed by a Tukey's multiple comparison test.a,b,cDifferent letters on the same column mean that values are significantly different (p