Evaluation of global gene expression changes in the larval intestines of PNGase-like (Pngl) mutant Drosophila

Mutations in Drosophila Pngl lead to structural and functional abnormalities in the larval intestine. Here we report using RNA-sequencing of Drosophila larval intestines to determine the effects of loss of Pngl on the transcriptome in this tissue. Overall design: Third instar Drosophila larvae with the following four different genotypes were synchronized at 96 hours after egg laying: 1. yellow white (y w control), 2. y w; Pngl[ex14]/CyO (Pngl heterozygous), 3. y w; Pngl[ex14]/Pngl[ex14] (Pngl homozygous), and 4. y w; Pngl[ex14]/Pngl[ex14]; PBac{Pngl[wt]Ch322-3O13}VK27 (Pngl homozygous rescued by one copy of a Pngl genomic transgene).