Assay conversion.

Amplicon sizes are approximate. Assays were performed in the HRMA reaction conditions and examined by gel electrophoresis and scored as follows: +++; both amplicons produced in equivalent amounts, +; both amplicons produced but one is favoured, −; one or both of the amplicons is not produced. Assays that did not score +++ were optimized with altered primer ratios and again assessed by electrophoresis. NT: not tested, NMP: Normalized melting peak, FDC: Fluorescence difference curve.