five

Yeast response to protein misfolding induced by azetidine-2-carboxylic acid (AZC)

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https://www.omicsdi.org/dataset/pride/PXD023668
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To identify proteins that interact with the yeast Tsa1 peroxiredoxin, we utilized an N-terminal Myc-tagged version of Tsa1 with a mutation in its resolving cysteineresidue (Tsa1-C171S) to trap and detect redox-dependent interactions. Yeast cells were left untreated, or treated with AZC or hydrogen peroxide prior to immunoprecipitation and mass spectrometry used to identify co-immunoprecipitating proteins.
创建时间:
2021-09-10
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