Ip, Wallace (2010) CIL:834, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were held at constant length and rigor was induced by permeabilization with glycerol. They were fixed in glutaraldehyde, quick-frozen by contact with a liquid helium-cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operating at 100 kev.

Images were recorded on Kodak 4489 film at 30,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

LEFT image of a stereo pair.

以甘油渗透化诱导僵直的方式，保持兔的腰大肌肌纤维长度恒定。随后，采用戊二醛进行固定，通过接触液氦冷却的铜块于 Heuser 型低温压机中进行快速冷冻，并在 Balzers 400 冷冻断裂机中进行冷冻蚀刻。样品随后在 24 度下以 Pt-C 进行旋转复制，并在操作于 100 keV 的 JEOL 100CX2 透射电子显微镜下进行观察。图像记录于 Kodak 4489 影胶片上，放大倍数为 30,000 倍。在 +5 度和 -5 度处拍摄立体对。使用 Nikon Coolscan 9000ED 扫描仪，以 20 微米像素间距对胶片进行扫描。立体对左侧图像。