A study on the impact of the absence of the NIP or NrpR gene on gene expression of Streptococcus salivarius K12 (SAL). A study on the impact of the absence of the NIP or NrpR gene on gene expression of Streptococcus salivarius K12 (SAL)

NIP and NrpR forms a pair for the transcriptional regulator in Streptococcus salivarius K12 (SAL). To explore the target genes regulated by the NIP-NrpR pair in Streptococcus salivarius K12 (SAL), we generated mutants in which the NIP or NrpR gene has been knocked down through complement recombination. Comparative transcriptome profiling of WT, ▲nrpR, and nip* mutant strains revealed that the sar BGC is the major regulatory target controlled by the NIP signaling pathway in SAL. Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different samples at the exponential growth phase of SAL. Comparative gene expression profiling analysis of RNA-seq data between wild type and mutant, total RNA was isolated from 3 independent growths (3 biological replicates)