Single-cell multi-omics decodes regulatory programs during development of mouse secondary palate

The abnormal perturbation in gene regulation during palatogenesis may lead to cleft palate. Here, we performed single-cell multiome sequencing (snRNA-seq and snATAC-seq simuteneously from same cells) from mouse secondary palate across 4 developmental stages. Overall design: Palatal shelves were isolated from time-mated C57BL/6J mice (Jackson Laboratory) at embryonic day (E) 12.5, E13.5, E14.0, and E14.5 and subjected to single-cell multiome sequencing. All mice were maintained in the animal facility of UTHealth. The protocol was approved by the Animal Welfare Committee (AWC) and the Institutional Animal Care and Use Committee (IACUC) of UT Health (AWC 19-0079).