Transcriptomic Data Reveals MYC as an Upstream Transcriptional Regulator in Laying Hen Pre-ovulatory Follicles using Ingenuity Pathway Analysis

To investigate the difference between the pre-ovulatory F5 and pre-recruitment follicular stages in laying hens and identify master regulators that control the processes in these two follicular stages. RNA was isolated from a group of 3-5 pre-recruitment follicles and the most recently recruited pre-ovulatory follicle (F5). After RNA isolation, follicles underwent RNA sequencing and differential gene expression analysis occurred. Follicles from two follicular stages from 12 month old hens were used. Total RNA was obtained folliclular tissue samples using TRIZol reagent and RNA-Sequencing occurred.