Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging [scRNA-Seq]

As the biggest organ of human body, skin and its appendages offer excellent experimental systems for aging studies. Skin wrinkling, hair greying and hair loss are among the most striking and observable traits of aging. However, the chronic changes and cell-to-cell variation accumulated during aging in different hair follicle lineages remain unexplored. Here we present a detailed analysis of scRNAseq and scATACseq data from young and aging animals. We found distinct cellular state changes of individual hair follicle populations. scRNA-Seq, 3 samples皮肤作为人体最大的器官，其附属结构为衰老研究提供了优质的实验模型系统。皮肤褶皱、毛发变白与脱发，是衰老最显著且易于观测的表型特征。然而，不同毛囊谱系在衰老过程中积累的慢性变化与细胞间异质性，仍有待深入探索。本研究针对年轻与衰老动物的单细胞RNA测序（scRNAseq）及单细胞转座酶可及性测序（scATACseq）数据开展了详尽分析，发现各毛囊细胞群体均呈现出独特的细胞状态变化。本次研究所用单细胞RNA测序（scRNA-Seq）样本共3例。