Marine microbial degradation of water-soluble crude oil components by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) with electrospray ionization negative mode (ESI(-)) direct infusion data

This dataset includes the semi-quantitative direct infusion data generated with a Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) with electrospray ionization negative mode (ESI(-)) from an experiment on marine microbial degradation of water-soluble polar crude oil components. Crude oil-derived polar compounds are receiving increasing attention due to their higher proportion in weathered oil, higher water solubility and potential harmful environmental impacts. However, these compounds are not amenable to GC-based analytical methods. Current knowledge on the characteristics and weathering patterns of oil-derived polar compounds relies on Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). Natural marine bacteria consortia were exposed to crude oil-derived compounds dissolved in seawater in a lab incubation study. Three sets of controls were used: (1) sterile seawater with oil-derived water-soluble compounds; (2) sterile seawater with natural marine bacteria and succinic acid, which was used as a simple carbon source instead of crude oil; and (3) seawater alone with natural marine bacteria. All incubation sets were supplemented with nutrients and kept in dark at room temperature for 14 days. This dataset includes semi-quantitative FT-ICR-MS direct infusion data, which focused on the molecular level chemical composition of the incubation media.

本数据集包含一项海洋微生物降解水溶性极性原油组分实验所产生的半定量直接进样质谱数据，该数据通过电喷雾电离负离子模式（ESI(-)）下的傅里叶变换离子回旋共振质谱（Fourier transform ion cyclotron resonance mass spectrometry, FT-ICR-MS）采集得到。原油衍生极性化合物因在风化原油中占比更高、水溶性更强且具备潜在环境危害，正受到学界日益广泛的关注。然而此类化合物无法采用基于气相色谱（Gas Chromatography, GC）的分析方法进行检测。当前学界关于原油衍生极性化合物的特征与风化模式的认知，均依托于傅里叶变换离子回旋共振质谱（FT-ICR-MS）技术。本研究通过实验室培养实验，将天然海洋细菌菌群暴露于溶解于海水的原油衍生化合物环境中。实验共设置三组对照组：（1）添加原油衍生水溶性化合物的无菌海水体系；（2）以琥珀酸作为替代原油的单一碳源、搭载天然海洋细菌的无菌海水体系；（3）仅搭载天然海洋细菌的天然海水体系。所有培养体系均补充营养盐，并于室温避光条件下培养14天。本数据集包含半定量直接进样傅里叶变换离子回旋共振质谱数据，聚焦于培养介质的分子级化学组成。