Whole ganglia RNA sequencing of C57 BL/6J mice

The study goal is to identify the gene expression profile of iBAT-related ganglia (SG/T1 & T3) and iWAT-related ganglia (T13/L1 & L2). RNA sequencing demonstrated clear separation of gene expression between brown adipose tissue related ganglia (SG and T3) and white adipose tissue related ganglia (T13/L1 and L2). Each sample type also showed clear separation from each other. No significant separation was observed in different genders. Overall design: All mice were fed with chow (LabDiet Rodent 5001) at room temperature. Ganglia SG, T3, T13/L1, L2 and ganglia fat were isolated from 4 male and 4 female C57 BL/6J mice, followed by RNA extraction. 40 samples were analyzed. There are 8 SG, 8 T3, 8 T13/L1, 8 L2, and 8 ganglia fat samples from 4 males and 4 females.