The miRNA expression profiles in exosomes derived from M0 macrophages (M0-Exo) and M2 macrophages (M2-Exo).

Macrophage-derived exosomes hold significant promise for clinical disease therapy. Macrophages can be categorized into several subtypes. The resting state is known as the M0 subtype, and the exosomes they secrete are termed M0-Exo. Macrophages can be polarized to the M2 subtype by combined induction with IL-4 and IL-13, and the exosomes derived from M2 macrophages are referred to as M2-Exo. miRNAs are important cargo within exosomes. Since both M0-Exo and M2-Exo exhibit therapeutic effects in inflammatory responses, we performed miRNA sequencing on the contents of M0-Exo and M2-Exo to investigate the underlying mechanisms of their therapeutic actions. Overall design: To ensure sufficient exosome yield, Raw264.7 cells were utilized for exosome isolation. The cells were divided into two groups. M0-Exo group: Raw264.7 cells were cultured under normal conditions for 24 hours, followed by a switch to serum-free medium. The supernatant was then collected for M0-Exo extraction.M2-Exo group: Raw264.7 cells were co-induced with 20 ng/ml IL-4 and 20 ng/ml IL-13 for 24 hours. Subsequently, the medium was replaced with serum-free medium, and the supernatant was collected for M2-Exo extraction.High-throughput sequencing was performed to analyze the miRNA expression profiles in both M0-Exo and M2-Exo.