RNAseq analysis of Hep3B cells upon overexpression of LINE1 retrotransposon encoded protein L1orf1p. RNAseq analysis of Hep3B cells upon overexpression of LINE1 retrotransposon encoded protein L1orf1p

L1 retrotransposons are known to get activated during hepatocarcinogenesis. We have developed inducible (TET-ON) overexpression of L1orf1 using piggybac vector in Hep3B cell line and a control TET-ON-empty cell line was also generated. Influence of L1orf1 overexpression on overall cellular transcritome was then analysed by RNAseq followed by pathway analysis. Overall, a number of key oncogeneic pathways are dysregulated upon L1orf1 overexpression is observed. Overall design: RNAseq was carried out for 2 cell lines derived from Hep3B cells (TET-ON-L1orf1 and TET-On-empty control) 72 hrs after doxycycline (1ug/ml) induction (RNA isolated from 3 independent cell pellets).