Single-nucleus RNA-seq results of AR-97Q and AR-24Q mice at 8 weeks of age.

To understand the early pathogenesis of motor neuron degeneration in spinal and bulbar muscular atrophy (SBMA), we conducted single-nucleus RNA-seq (snRNA-seq) on the spinal cords of AR-97Q and AR-24Q mice at 8 weeks of age. REST target genes were up-regulated in motor neurons of AR-97Q mice. Overall design: snRNA-seq was conducted on spinal cord samples from AR-97Q and AR-24Q mice at an early stage (8 weeks of age). Spinal cords from three mice were pooled per sample for homogenization and nuclear isolation.