Low Coverage Whole-Genome Sequencing of Mouse Normal, PanIN Lesions, Tumor and Metastatic Pancreatic Ductal Adenocarcinoma Organoids

This study used low coverage whole-genome sequencing to examine copy number variations between matched murine tumor- and metastasis-derived mouse pancreatic ductal adenocarcinoma (PDAC) cells grown as three-dimensional, organoid cultures. Murine PanIN lesions and normal pancreas cells grown as organoid cultures have also been included. The study analyzed 16 organoid lines derived from matched primary PDAC tumors and PDAC metastases from 6 KPC (KrasLSL-G12D; Trp53LSL-R172H; Pdx1-Cre) mice.