Whole body Knockout of PC-TP (Pctp-/-), Hepatic whole RNAseq comparing Wild Type (WT) and knock out mic (KO) as well as Chow Fed and Methionine and choline depleted (MCD) fed mice.

Previous studies have used this model to show that PC-TP has a protective effect when challenged with a methionine and choline depleted diet, known to induce NAFLD. We performed transcription factor binding experiments and show that PPARs are dysregulated in the contect of PC-TP depletion We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 Mice for WT and KO fed either normal chow or methionine and choline depleted chow. Comparative gene expression profiling analysis of RNA-seq data for WT and KO Livers fed MCD or Chow diet既往研究借助该实验模型证实，PC-TP在小鼠暴露于可诱导非酒精性脂肪性肝病（Non-Alcoholic Fatty Liver Disease, NAFLD）的蛋氨酸胆碱缺乏（Methionine and Choline Depleted, MCD）饮食时，具有保护作用。我们开展了转录因子结合实验，结果显示在PC-TP缺失的背景下，过氧化物酶体增殖物激活受体（Peroxisome Proliferator-Activated Receptors, PPARs）表达失调。随后，我们利用3只野生型（Wild Type, WT）与3只基因敲除型（Knock Out, KO）小鼠的RNA测序（RNA-seq）数据开展基因表达谱分析，这些小鼠分别饲喂普通饲料或MCD饲料。本研究针对饲喂MCD饲料或普通饲料的野生型与基因敲除型小鼠肝脏的RNA测序数据，完成了对比基因表达谱分析。