Additional file 17 of Cisplatin exposure alters tRNA-derived small RNAs but does not affect epimutations in C. elegans

Additional file 17: Table S7. Stepwise analysis of association of gene expression changes and 22G-RNAs epimutations in the different exposure conditions. To test the association between 22G-RNAs epimutations and gene expression epimutations, we first looked at all genes as a background regardless of whether they had expression change or not. Then, the proportion of genes with gene expression epimutation was compared to the proportion of genes which had 22G-RNAs epimutation. The overlap, in which genes had both expression changes and 22G-RNA epimutations, was calculated (Test 1). In a second time, only genes with gene expression epimutation were selected and among these genes, the proportion of genes with inherited expression changes was compared with the proportion of genes with 22G-RNA epimutations and the overlap was calculated (Test 2). In third, among genes with genes expression changes, the proportion of ones with inherited expression change was compared with the proportion of genes with simultaneous 22G-RNA epimutation. The overlap of genes with both conditions was calculated (Test 3) Finally, this third analysis was repeated but among genes with BOTH gene expression change and 22G-RNA epimutation (Test 4). Odds ratios and p-values for each test and in each condition were calculated with Fisher’s Exact Test. Cells in yellow indicate significantly increased odds of association, cells in grey indicate no significant association.