Pattern of recognition of proteins by the monoclonal antibodies used in specificity studies by immunoblot.

Pb339 CYA - cytoplasmic yeast antigen of Paracoccidioides brasiliensis; Pb (f) - filtrated antigen of P. brasiliensis; Af (son) – sonicated antigen of Aspergillus fumigatus; Af (f) - filtrated antigen of A. fumigatus; Bd CYA - cytoplasmic yeast antigen of Blastomyces dermatitidis. Ss CYA - cytoplasmic yeast antigen of Sporothrix schenckii; Mt (son) - sonicated antigen of Mycobacterium tuberculosis. Ag rM - recombinant M antigen of Histoplasma capsulatum; CYA Hc - cytoplasmic yeast antigen of H. capsulatum; pHMIN - purified and periodate treated histoplasmin. The numbers listed show the molecular weight of the protein recognized when probed with each monoclonal antibody. Absence of reactivity is indicated by an ‘X’.