AMPKα1 and α2 knockout and usage of alternative polyadenylation sites

Role of alternative polyadenylation in muscle development remains largely undetermined. Our WTTS-seq approach to capture 3'-end of RNAs clearly revealed alternaitve polyadenylation events responsible for reduced muscle mass in AMPK α1, but increased muscle mass in AMPK α2 knockout mice. Total RNA samples obtained from muscle tissue of knockout (KO) mice and wild-type (WT) mice were used to construct WTTS-seq libraries. Eight WTTS-seq libraries including two AMPKα1 KO mice, two AMPK α2 KO mice, two AMPKα1 WT mice and two AMPK α2 WT mice were involved in this submission.