Screening the crucial exosome miRNA in the human serum

With the increasing aging population, the incidence of osteoporosis is increasing year by year. Fractures caused by osteoporosis are one of the main causes of disability and death in elderly people. Therefore, the purpose of this study is to recruit postmenopausal osteoporosis and healthy patients, collect their blood and isolate serum, screen for miRNAs related to osteoporosis through exosomal miRNA sequencing and bioinformatics analysis, validate these molecules through experiments, and finally screen for key miRNAs. 1. Recruit 10 postmenopausal female patients, including 5 healthy controls and the other 5 patients with osteoporosis; 2. Collect whole blood, let it stand at 4 ℃ for 2-3 hours, and then isolate the serum; 3. Extract serum exosomes using the reagent kit method; 4. Extract extracellular RNA and perform sequencing; 5. Bioinformatics screening of key miRNAs in sequencing data; 6. Verify miRNA through in vitro and in vivo experiments