glutamate + tRNA(Glu) + ATP => Glu-tRNA(Glu) + AMP + pyrophosphate

Multifunctional EPRS (cytosolic glutamyl prolyl tRNA synthetase) catalyzes the reaction of glutamate, tRNA(Glu), and ATP to form Glu tRNA(Glu), AMP, and pyrophosphate. The same enzyme also catalyzes the charging of tRNA(Pro) with proline. The enzyme is found in the cell as a component of the mutienzyme aminoacyl tRNA synthetase complex (Kaiser et al. 1994).

多功能EPRS（细胞质谷氨酰丙氨酰tRNA合成酶）催化谷氨酸、tRNA(Glu)和ATP反应生成谷氨酰tRNA(Glu)、AMP和焦磷酸。同一酶亦催化tRNA(Pro)的丙氨酸装载。该酶作为多酶复合体氨基酸酰基tRNA合成酶复合物（Kaiser等，1994年）的组成部分存在于细胞中。