Alternative splicing analysis of different growth stages of O. sinensis

ASprofile (version b-1.0.4, http://ccb.jhu.edu/software/ASprofile/ was used to detect the splice junction sites and provided information about boundaries and combinations of different exons in a transcript, and then total splice junction sites of the same gene were used to distinguish the type of its alternative splicing (AS) event. Using rMATs (version 4.0.3, http://rnaseq-mats.sourceforge.net/), the number of differentsplicing events (DASs) and different splicing genes (DSGs) were compared between different growth stages and FDR ≤ 0.05 as statistically significant.