Differential Ago-RIP-Seq for the identification of miR-375 targets in prostate cancer cells. Differential Ago-RIP-Seq for the identification of miR-375 targets in prostate cancer cells
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB9759
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资源简介：
The prostate cancer cell line PC-3 was transfected with pre-miR-375 or control. After 48 hours the cells were lysed and immunoprecipitation was performed using anti-panAgo (...) or isotype anti-IgG antibodies overnight. RNA of total lysates and immunoprecipitation fractions was extracted using miRNeasy kit (Qiagen) and libraries were generated using the Stranded Total RNA Sample Prep Kit (Takara Clontech). The experiment was performed in order to identify potential targets of miR-375 in prostate cancer.
创建时间：
2017-02-01
