The sequencing result of the coding region of RADA-16 in the C-terminus of 96R. This result indicated the coding sequence of RADA-16 in cloning vector pMD19-T-hELP96-RADA-16. (AB1 292 kb)
We show that the amber termination codon UAG can initiate protein synthesis in Escherichia coli. We mutated the initiation codon AUG of the chloramphenicol acetyltransferase (CAT) gene to UAG (CATam1)