Bifidobacterium longum subsp. infantis transcriptomes in response to various nitrogen sources. Bifidobacterium longum subsp. infantis transcriptomes in response to various nitrogen sources

The purpose of this project was to determine the whole transcriptome response of Bifidobacterium longum subsp. infantis to human milk urea compared to complex nitrogen and L-cysteine. Overall design: RNA was isolted and sequenced from three strains of Bifidobacterium longum subsp. infantis grown on modified MRS supplemented with urea, complex nitrogen and L-cysteine as a primary nitrogen source. Biological duplicate was performed for each bacteria strain with each treatment.